Pleurotus pulmonarius was cultivated in three different environmental conditions. in ambient indoor environment (System 1). in humidifying without ventilation (System 2) and in humidifying with ventilation (System 3) to analyse the major nutritional contents. Sawdust was the main substrate for all the cultivation systems. https://www.marcelovicente.com/product-category/eyelash-and-eyebrow-dye/